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Plant Science Today (PST)

Research Articles

Vol. 10 No. 1 (2023)

Effect of cadmium stress on seed germination and seedling morpho-physiological growth parameters of barnyard millet (Echinochloa frumentacea Link)

DOI
https://doi.org/10.14719/pst.2012
Submitted
20 July 2022
Published
23-12-2022 — Updated on 12-01-2023
Versions

Abstract

Cadmium (Cd) is a heavy metal, which is seen in the contaminated soils and severely affects the growth and development of plants in recent years. The study on the seed germination and morpho-physiological growth characteristics of barnyard millet (Echinochloa frumentacea) cultivar CO (KV) 2 treated with different concentrations (50, 100, 150, 200, and 250 mg/kg of soil) of Cd were evaluated at 15th, 30th, and 45th day of interval. The findings of this research demonstrate that the maximum dosage of Cd (250 mg/kg of soil) affects the germination percentage (65%) of barnyard millet. Seedling vigor index has been negatively influences a drop in germination percentage. Increasing concentrations of Cd reveals the growth of root and shoot length and the quantity of fresh and dry weight affected. The phytotoxicity percentage of roots and shoots also increases with increasing concentrations of Cd, whereas the tolerance index level decreases with increasing concentrations of Cd. In root and shoot, the relative growth index was reduced in higher concentration of Cd. The relative water content remains high in the initial stages of leaf development and declines when the leaf matures. From this study, it was found that the increase in the concentration of Cd leads to decrease the germination percentage and morpho-physiological growth parameters as compared to control.

References

  1. Alloway BJ. Heavy metal in soils. New York: John Wiley & Sons; 1990.
  2. Raskin J, Kumar PBAN, Dushenkov S, Salt DE. Bioconcentration of heavy metals by plants. Curr Opin Biotechnol. 1994;5:285-90. https://doi.org/10.1016/0958-1669(94)90030-2
  3. Shen Z, Li X, Wang C, Chen H, Chua H. Lead phytoextraction from contaminated soil with high biomass plant species. J Environ Qual. 2002;31:1893-900. https://doi.org/10.2134/jeq2002.1893
  4. Ali H, Khan E, Sajad MA. Phytoremediation of heavy metals—concepts and applications. Chemosphere. 2013;91:869-81. https://doi.org/10.1016/j.chemosphere.2013.01.075
  5. Nagajyoti PC, Lee KD, Sreekanth TVM. Heavy metals, occurrence and toxicity for plants: a review. Environ Chem Lett. 2010;8:199-216. https://doi.org/10.1007/s10311-010-0297-8
  6. Ling W, Shen Q, Gao Y, Gu X, Yang Z. Use of bentonite to control the release of copper from contaminated soils. Aust J Soil Res. 2007;45:618-23. https://doi.org/10.1071/SR07079
  7. Cirlaková A. Heavy metals in the vascular plants of Tatra mountains. Oecol Mont. 2009;18:23-6. Available from: https://om.vuvb.uniza.sk/index.php/OM/article/view/210
  8. Flora SJS, Mittal M, Mehta A. Heavy metal induced oxidative stress & its possible reversal by chelation therapy. Ind J Med Res. 2008;128:501-23. Available from: https://pubmed.ncbi.nlm.nih.gov/19106443
  9. Shah K, Dubey RS. A 18 kDa cadmium inducible protein complex from rice: its purification and characterization from rice (Oryza sativa L.) roots tissues. J Plant Physiol. 1998;152:448-54. Available from: https://link.springer.com/chapter/10.1007/978-3-7643-8554-5_30
  10. Moya JL, Ros R, Picazo I. Influence of cadmium and nickel on growth, net photosynthesis and carbohydrate distribution in rice plants. Photosynth Res. 1993;36:75-80. https://doi.org/10.1007/BF00016271
  11. Glass DJ. Economic potential of phytoremediation. In: Raskin I, Ensley BD, editors. Phytoremediation of toxic metals: using plants to clean up the environment. New York: John Wiley and Sons; 2000. p. 15-31.
  12. Das PS, Samantaray S, Rout GR. Studies on cadmium toxicity in plant: a review. Environ Pollut. 1997;98:29-36. https://doi.org/10.1016/S0269-7491(97)00110-3
  13. Ahmad P, Jaleel CA, Salem MA, Nabi G, Sharma S. Roles of enzymatic and nonenzymatic antioxidants in plants during abiotic stress. Crit Rev Biotechnol. 2010;30:161-75. https://doi.org/10.3109/07388550903524243
  14. Asopa PP, Bhatt R, Sihag S, Kothari SL, Kachhwaha S. Effect of cadmium on physiological parameters of cereal and millet plants—a comparative study. Int J Phytoremediation. 2017;19(3):225-30. https://doi.org/10.1080/15226514.2016.1207608
  15. Abe T, Fukami M, Ogasawara M. Cadmium accumulation in the shoots and roots of 93 weed species. Soil Sci Plant Nutr. 2008;54:566. https://doi.org/10.1111/j.1747-0765.2008.00288.x
  16. Sood S, Khulbe R, Kumar RA, Agrawal PK, Upadhyaya H. Barnyard millet global core collection evaluation in the submountain Himalayan region of India using multivariate analysis. Crop J. 2015;3:517-25. http://dx.doi.org/10.1016/j.cj.2015.07.005
  17. de Wet JMJ, Rao KP, Mengesha MH, Brink DE. Domestication of mawa millet (Echinochloa colona). Econ Bot. 1983;37:283-91. https://doi.org/10.1007/BF02858883
  18. Sampath TV, Razvi SM, Singh DN, Bandale KV. Small millets in Indian agriculture. In: Seetaram A, Riley KU, Hariyana G, editors. Small millets in global agriculture. New Delhi: Oxford and IBH Publishing Co. Pvt.; 1986. p. 33-43.
  19. Channappagoudar BB, Hiremath S, Bradar NR, Koti RV, Bharamagoudar TD. Influence of morpho-physiological and biochemical traits on the productivity of barnyard millet. Karnataka J Agric Sci. 2008;20:477-80. Available from: http://14.139.155.167/test5/index.php/kjas/article/viewFile/888/881
  20. Devi PB, Vijayabharathi R, Sathyabama S, Malleshi NG, Priyadarisini VB. Health benefits of finger millet (Eleusine coracana L.) polyphenols and dietary fiber: a review. J Food Sci Technol. 2014;51:1021-40. https://doi.org/10.1007/s13197-011-0584-9
  21. Kumar KK, Parameshwaran PK. Characterization of storage protein from selected varieties of foxtail millet (Setaria italica (L) Beauv). J Sci Food Agric. 1998;77:535-42. https://doi.org/10.1002/(SICI)1097-0010(199808)77:4<535::AID-JSFA77>3.0.CO;2-G
  22. Veena B, Chimmad BV, Naik RK, Shantakumar G. Physico-chemical and nutritional studies in barnyard millet. Karnataka J Agric Sci. 2005;18:101-5. Available from: http://14.139.155.167/test5/index.php/kjas/article/viewFile/3019/3248
  23. Kulkarni LR, Naik RK, Katarki PA. Chemical composition of minor millets. Karnataka J Agric Sci. 1992;5:255-8. Available from: http://14.139.155.167/test5/index.php/kjas/article/viewFile/6233/6535
  24. Venkatesan S, Sujatha K, Geetha R, Senthil N. Standardization of duration for accelerated ageing in barnyard millet cv. CO2 and MDU1. Electr J Plant Breed. 2018;9:12334-8. http://doi.org/10.5958/0975-928x.2018.00152.7
  25. Bewley JD, Black M. Physiology and biochemistry of seeds in relation to germination. Vol. 2. Viability, dormancy, and environmental control. New York: Springer Science & Business Media; 2012.
  26. Dhindwal AS, Lather BPS, Singh J. Efficacy of seed treatments on germination. Seedling emergence and vigour of cotton (Gossypium hirsutum) genotypes. Seed Res. 1991;19:59-61. Available from: https://eurekamag.com/research/031/199/031199716.php
  27. O'Kelly B. (2005). Oven-drying characteristics of soils of different origins. Drying Technol. 2005;23:1141-9. https://doi.org/10.1081/DRT-200059149
  28. Chou CH, Lin HJ. Autointoxication mechanism of Oryza sativa L.: phytotoxic effects of decomposing rice residues in soil. J Chem Ecol. 1976;2:353-67. https://doi.org/10.1007/BF00988282
  29. Baker AJM, Reeves RD, Hajar AM. Heavy metal accumulation and tolerance in British population of the metallophyte Thlaspi caerulescens J. & C. Presl (Brassicaceae). New Phytol. 1994;127:61-8. https://doi.org/10.1111/j.1469-8137.1994.tb04259.x
  30. Paliouris G, Hutchinson TC. Arsenic, cobalt and nickel tolerances in two populations of Silene vulgaris (Moench) Garcke from Ontario, Canada. New Phytol. 1991;117:449-59. https://doi.org/10.1111/j.1469-8137.1991.tb00009.x
  31. Weatherley P. Studies in the water relations of the cotton plant. New Phytol. 1950;49:81-97. https://doi.org/10.1111/j.1469-8137.1950.tb05146.x
  32. Aydinalp C, Marinova S. The effects of heavy metals on seed germination and plant growth on alfalfa plant (Medicago sativa). Bulg J Agric Sci. 2009;15:347-50. Available from: http://www.agrojournal.org/15/04-11-09.pdf
  33. He J, Ren Y, Zhu C, Jiang D. Effects of cadmium stress on seed germination, seedling growth and seed amylase activities in rice (Oryza sativa). Rice Sci. 2008;15:319-25. https://doi.org/10.1016/S1672-6308(09)60010-X
  34. Raziuddin, Farhatullah, Hassan G, Akmal M, Salim Shah S, Mohammad F, et al. Effects of cadmium and salinity on growth and photosynthesis parameters of Brassica species. Pak J Bot. 2011;43:333-40. Available from: http://www.pakbs.org/pjbot/PDFs/43(1)/PJB43(1)333.pdf
  35. Titov AF, Talanova VV, Boeva NP. Growth responses of barley and wheat seedlings to lead and cadmium. Biol Plant. 1996;38:431-6. Available from: https://www.bp.ueb.cas.cz/pdfs/bpl/1996/03/19.pdf
  36. Peralta JR, Gardea-Torresdey JL, Tiemann KJ, Gomez E, Arteaga S, Rascon E, et al. Uptake and effects of five heavy metals on seed germination and plant growth in alfalfa (Medicago sativa L.). Bull Environ Contam Toxicol. 2001;66:727-34. https://doi.org/10.1007/s001280069
  37. Zeid IM. Responses of Phaseolus vulgaris to chromium and cobalt treatment. Biol Plant. 2001;44:111-5. https://doi.org/10.1023/A:1017934708402
  38. Marcos FJ. Seed vigor testing: an overview of the past, present and future perspective. Sci Agric. 2015;72:363-74. https://doi.org/10.1590/0103-9016-2015-0007
  39. Barceló J, Poschenrieder CH. Plant water relations as affected by heavy metal stress: a review. J Plant Nutr. 1990;13:1-37. https://doi.org/10.1080/01904169009364057
  40. Leita L, De Nobili M, Mondini C, Baca-Garcia MT. Response of leguminosae to cadmium exposure. J Plant Nutr. 1993;16:2001-12. https://doi.org/10.1080/01904169309364670
  41. Azevedo H, Gomes C, Pinto G, Fernandes J, Loureiro S, Santos C. Cadmium effects on sunflower growth and photosynthesis. J Plant Nutr. 2005;28:2211-20. https://doi.org/10.1080/01904160500324782
  42. Zhang F, Shi W, Jin Z, Shen Z. Response of antioxidative enzymes in cucumber chloroplasts to toxicity. J Plant Nutr. 2003;26:1779-88. https://doi.org/10.1081/PLN-120023282
  43. Mondal NK, Das C, Roy S, Datta JK, Banerjee A. Effect of varying cadmium stress on chickpea (Cicer arietinum L) seedlings: an ultrastructural study. Annals Environ Sci. 2013;7:59-70. Available from: http://hdl.handle.net/2047/d20018674
  44. Metwally A, Safronova VI, Belimov AA, Dietz KJ. Genotypic variation of the response to cadmium toxicity in Pisum sativum L. J Exp Bot. 2004;56:167-78. https://doi.org/10.1093/jxb/eri017
  45. Shiyab S. Morphophysiological effects of chromium in sour orange (Citrus aurantium L.). HortScience. 2019;54:829-34. https://doi.org/10.21273/HORTSCI13809-18
  46. Gallego SM, Pena LB, Barcia RA, Azpilicueta CE, Iannone MF, Rosales EP, et al. Unravelling cadmium toxicity and tolerance in plants: insight into regulatory mechanisms. Environ Exp Bot. 2012;83:33-46. https://doi.org/10.1016/j.envexpbot.2012.04.006
  47. Dai H, Shan C, Jia G, Lu C, Yang T, Wei A. Cadmium detoxification in Populus ? canescens. Turk J Bot. 2013;37:950-5. https://doi.org/10.3906/bot-1110-9
  48. Nikoli? N, Zori? L, Cvetkovi? I, Pajevi? S, Borišev M, Orlovi? S, et al. Assessment of cadmium tolerance and phytoextraction ability in young Populus deltoides L. and Populus ? euramericana plants through morpho-anatomical and physiological responses to growth in cadmium enriched soil. iFor Biogeosci For. 2017;10:635-44. https://doi.org/10.3832/ifor2165-010
  49. Pereira AS, Cortez PA, Almeida AAF, Prasad MNV, França MGC, Cunha M, et al. Morphology, ultrastructure, and element uptake in Calophyllum brasiliense Cambess. (Calophyllaceae J. Agardh) seedlings under cadmium exposure. Environ Sci Pollut Res. 2017;24:15576-88. https://doi.org/10.1007/s11356-017-9187-y
  50. Mehes-Smith M, Nkongolo K, Cholewa E. Coping mechanisms of plants to metal contaminated soil. In: Silvern S, editor. Environmental change and sustainability. Vol. 54. Rijeka, Croatia: In Tech; 2013. p. 53-90.
  51. Hasanuzzaman M, Nahar K, Anee TI, Fujita M. Glutathione in plants: biosynthesis and physiological role in environmental stress tolerance. Physiol Mol Biol Plants. 2017;23:249-68. https://doi.org/10.1007/s12298-017-0422-2
  52. Hernández LE, Sobrino-Plata J, Montero-Palmero MB, CarrascoGil S, Flores-Cáceres ML, Ortega-Villasante C, et al. Contribution of glutathione to the control of cellular redox homeostasis under toxic metal and metalloid stress. J Exp Bot. 2015;66:2901-11. https://doi.org/10.1093/jxb/erv063
  53. Fernández R, Fernández-Fuego D, Bertrand A, González A. Strategies for Cd accumulation in Dittrichia viscosa (L.) Greuter: role of the cell wall, non-protein thiols and organic acids. Plant Physiol Biochem. 2014;78:63-70. https://doi.org/10.1016/j.plaphy.2014.02.021
  54. Parrotta L, Guerriero G, Sergeant K, Cai G, Hausman JF. Target or barrier? The cell wall of early- and later-diverging plants vs cadmium toxicity: differences in the response mechanisms. Front Plant Sci. 2015;2:133. https://doi.org/10.3389/fpls.2015.00133
  55. Weigel HJ, Jäger HJ. Subcellular distribution and chemical forms of cadmium in bean plants. Plant Physiol. 1980;65:480-2. https://doi.org/10.1104/pp.65.3.480
  56. Farooq M, Wahid A, Kobayashi N, Fujita D, Basra SMA. Plant drought stress: effects, mechanisms and management. Agron Sustain Dev. 2009;29:185-212. https://doi.org/10.1051/agro:2008021
  57. Lugojan C, Ciulca S. Evaluation of relative water content in winter wheat. J Hort For Biotechnol. 2011;15:173-7. Available from: https://journal-hfb.usab-tm.ro/romana/2011/Lista%20lucrari_2011%20PDF/JHFB_15(2)_PDF/32Lugojan%20Cristian.pdf
  58. Liu Y, Fiskum G, Schubert D. Generation of reactive oxygen species by mitochondrial electron transport chain. J Neurochem. 2002;80:780-7. https://doi.org/10.1046/j.0022-3042.2002.00744.x

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