Quality control of marketed herbal products of Asparagus racemosus  Willd. through high performance thin layer chromatography (HPTLC) analysis

Bibhuti Bhusan Champati; Bhuban Mohan Padhiari; Asit Ray; Sudipta Jena; Ambika Sahoo; Tirthabrata  Sahoo; Pratap Chandra Panda; Sanghamitra Nayak

doi:10.14719/pst.2206

Authors

Bibhuti Bhusan Champati Center for Biotechnology, Siksha ‘O’ Anusandhan (Deemed to be) University, Kalinga Nagar, Bhubaneswar, Odisha-751 003, India https://orcid.org/0000-0003-2792-0280
Bhuban Mohan Padhiari Center for Biotechnology, Siksha ‘O’ Anusandhan (Deemed to be) University, Kalinga Nagar, Bhubaneswar, Odisha-751 003, India https://orcid.org/0000-0002-2371-639X
Asit Ray Center for Biotechnology, Siksha ‘O’ Anusandhan (Deemed to be) University, Kalinga Nagar, Bhubaneswar, Odisha-751 003, India https://orcid.org/0000-0002-6111-9529
Sudipta Jena Center for Biotechnology, Siksha ‘O’ Anusandhan (Deemed to be) University, Kalinga Nagar, Bhubaneswar, Odisha-751 003, India https://orcid.org/0000-0003-4770-6185
Ambika Sahoo Center for Biotechnology, Siksha ‘O’ Anusandhan (Deemed to be) University, Kalinga Nagar, Bhubaneswar, Odisha-751 003, India https://orcid.org/0000-0002-5016-3735
Tirthabrata Sahoo Department of Botany, Science College, Konkarada, Ganjam, Odisha-761 144, India https://orcid.org/0000-0001-6984-8999
Pratap Chandra Panda Center for Biotechnology, Siksha ‘O’ Anusandhan (Deemed to be) University, Kalinga Nagar, Bhubaneswar, Odisha-751 003, India https://orcid.org/0000-0001-6984-8999
Sanghamitra Nayak Center for Biotechnology, Siksha ‘O’ Anusandhan (Deemed to be) University, Kalinga Nagar, Bhubaneswar, Odisha-751 003, India https://orcid.org/0000-0001-9038-261X

DOI:

https://doi.org/10.14719/pst.2206

Keywords:

Asparagus racemosus, Shatavarin IV, HPTLC, Quality control, Adulteration, Herbal products.

Abstract

Asparagus racemosus Willd. is a valuable medicinal plant which is used all over the world. There are several marketed products of A. racemosus. The high demand for this herb has increased the risk of adulteration in its commercial products. The adulterated herbal products might pose serious ill effects on health. Therefore, it is necessary to check the quality of marketed products in terms of the presence of their major bioactive compounds. The present study aimed to carry out the qualitative and quantitative analysis of Shatavarin IV in marketed products of A. racemosus through a validated high performance thin layer chromatography (HPTLC) method. Ten marketed products were analysed and all of them had shown the presence of Shatavarin IV which was quantified. The identification and quantification were done by taking a standard Shatavarin IV as reference. The Shatavarin IV was detected at Rf 0.4±0.05 and showed maximum absorption at 425 nm. The Shatavarin IV was quantified using a 6-point calibration curve having a standard deviation of 3.89 % with an R2 value of 0.9968. The amount of Shatavarin IV varied between 1.47±0.25 to 2.69±0.51 mg/g on a dry weight basis which is a normal range in the raw plant materials. Thus, the present findings would be a simple, reliable and cost-effective method for the quality determination of herbal products of A. racemosus. The developed HPTLC chromatograms would serve as a reference for the quality assessment of commercial products of A. racemosus in future.

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