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Plant Science Today (PST)

Research Articles

Early Access

Evaluation of Phenolic Content, Anti-oxidant and modulation of blood indices of Pavetta crassipes

DOI
https://doi.org/10.14719/pst.2453
Submitted
21 February 2023
Published
10-07-2023
Versions

Abstract

Introduction
Pavetta crassipes is a medicinal plant with various documented biological activities. It has a folkloric use as an anti-viral therapy and pharmacologically documented anti-microbial activity. However, its immune modulatory property has not been explored. The study is designed to evaluate the phytochemical profile as well as the anti-oxidant and immunomodulatory potential of the methanol extract of Pavetta crassipes leaf.

Methodology
In vitro antioxidants activity was determined by nitric oxide inhibitory assay. phenolic and flavonoid content of the extract was also determined. The immunomodulatory activity was evaluated using cyclophosphamide induced immunomodulation model in mice. Phytochemical evaluation of leaf extract was done using HPLC analyses. Results were expressed as the mean ± SEM, IC50 value for the antioxidant activity was determined, One-way ANOVA was used for differences in mean followed by Dunnett’s test for multiple comparison for the immunomodulatory activity. To test for level of significance, P< 0.05 was considered significant.

Results
The extract had phenolic and flavonoid contents of 17.6 ±0.1 mg/GAE/g and 8.4 ± 0.3 mg/QE/g respectively. The extract also showed nitric oxide inhibitory antioxidant activity with IC50 of 2.3± 0.4 while gallic and ascorbic acid had IC50 of 1.6 ±0.1 and 8.0 ±0.6 respectively. P. crassipes leaf extract indicated a significant (p<0.05) countering influence to cyclophosphamide induced decrease in total white blood count, differential leucocyte counts and red blood count. HPLC analysis indicated the presence of phenolic compounds such a rutin, quercetin and chlorogenic acid.

Conclusion
The phytochemical profile, anti-oxidant and immunomodulatory properties of the methanol extract of P. crassipes leaf as evaluated in this study corroborates and justifies its folkloric use.

References

  1. Mushtaq S, Abbasi BH, Uzair B, Abbasi R. Natural products as reservoirs of novel therapeutic agents. EXCLI Journal. 2018;17:420. doi: 10.17179/excli2018-1174
  2. Shaikh AS, Thomas AB, Chitlange SS. Herb–drug interaction studies of herbs used in treatment of cardiovascular disorders—A narrative review of preclinical and clinical studies. Phytotherapy Research. 2020;34(5):1008-26. doi.org/10.1002/ptr.6585
  3. Behl T, Kumar K, Brisc C, Rus M, Nistor-Cseppento DC, Bustea C et al. Exploring the multifocal role of phytochemicals as immunomodulators. Biomedicine & Pharmacotherapy. 2021;133:110959. doi.org/10.1016/j.biopha.2020.110959
  4. Muhammad Q, Jang Y, Kang SH, Moon J, Kim WJ, Park H. Modulation of immune responses with nanoparticles and reduction of their immunotoxicity. Biomaterials Science. 2020;8(6):1490-501. https://doi.org/10.1039/C9BM01643K
  5. Al-Hariri M. Immune's-boosting agent: Immunomodulation potentials of propolis. Journal of Family & Community Medicine. 2019;26(1):57. doi: 10.4103/jfcm.JFCM_46_18
  6. Feng X, Xu W, Li Z, Song W, Ding J, Chen X. Immunomodulatory nanosystems. Advanced Science. 2019;6(17):1900101. https://doi.org/10.1002/advs.201900101
  7. Alijotas-Reig J, Esteve-Valverde E, Belizna C, Selva-O'Callaghan A, Pardos-Gea J, Quintana A et al. Immunomodulatory therapy for the management of severe COVID-19. Beyond the anti-viral therapy: A comprehensive review. Autoimmunity Reviews. 2020;19(7):102569. https://doi.org/10.1016/j.autrev.2020.102569
  8. Mohan MR. Indian medicinal plants used as immunomodulatory agents: A review. International Journal of Green Pharmacy (IJGP). 2019;13(04). https://doi.org/10.22377/ijgp.v13i04.2700
  9. Suslov A, Wieland S, Menne S. Modulators of innate immunity as novel therapeutics for treatment of chronic hepatitis B. Current Opinion in Virology. 2018;30:9-17. https://doi.org/10.1016/j.coviro.2018.01.008
  10. Sordillo LM, Aitken SL. Impact of oxidative stress on the health and immune function of dairy cattle. Veterinary Immunology and Immunopathology. 2009;128(1-3):104-09. https://doi.org/10.1016/j.vetimm.2008.10.305
  11. Park JS, Chyun JH, Kim YK, Line LL, Chew BP. Astaxanthin decreased oxidative stress and inflammation and enhanced immune response in humans. Nutrition & Metabolism. 2010;7(1):1-10. https://doi.org/10.1186/1743-7075-7-18
  12. Iddir M, Brito A, Dingeo G, Fernandez Del Campo211 SS, Samouda H, La Frano MR et al. Strengthening the immune system and reducing inflammation and oxidative stress through diet and nutrition: considerations during the COVID-19 crisis. Nutrients. 2020;12(6):1562. https://doi.org/10.3390/nu12061562
  13. Bariweni MW, Ozolua RI. Neuropharmacological effects of the aqueous leaf extract and fractions of Pavetta crassipes (K. Schum) Rubiaceae in mice. Journal of Pharmacy & Pharmacognosy Research. 2017;5(5):278-87.
  14. Bello IA, Ndukwe GI, Audu OT, Habila JD. A bioactive flavonoid from Pavetta crassipes K. Schum. Organic and Medicinal Chemistry Letters. 2011;1(1):1-5. https://doi.org/10.1186/2191-2858-1-14
  15. Sanon S, Ollivier E, Azas N, Mahiou V, Gasquet M, Ouattara C et al. Ethnobotanical survey and in vitro antiplasmodial activity of plants used in traditional medicine in Burkina Faso. Journal of Ethnopharmacology. 2003;86(2-3):143-47. https://doi.org/10.1016/S0378-8741(02)00381-1
  16. Amos S, Akah PA, Binda L, Enwerem NM, Ogundaini A, Wambebe C et al. Hypotensive activity of the ethanol extract of Pavetta crassipes leaves. Biological and Pharmaceutical Bulletin. 2003;26(12):1674-80. https://doi.org/10.1248/bpb.26.1674
  17. Baldé ES, Megalizzi V, Traoré MS, Cos P, Maes L, Decaestecker C et al. In vitro antiprotozoal, antimicrobial and antitumor activity of Pavetta crassipes K. Schum leaf extracts. Journal of Ethnopharmacology. 2010;130(3):529-35. https://doi.org/10.1016/j.jep.2010.05.042
  18. Ibekwe NN, Boshoff H, Randle J, Adesomoju AA, Barry III CE, Okogun JI. Chemical constituents and antimycobacterial studies of the leaf extracts of Pavetta crassipes K. Schum. Tropical Plant Research. 2018;5(1):88-95.
  19. Kotwal S, Patil D, Patil H. Reported herbal immunomodulators for better health: An overview. Wrld J of Pharm Res. 2020;9(612):37.
  20. Kshirsagar SG, Rao RV. Antiviral and immunomodulation effects of Artemisia. Medicina. 2021;57(3):217. https://doi.org/10.3390/medicina57030217
  21. Mustapha Y, Bala M. Antimicrobial activity of leaf extracts of Pavetta crassipes (hutch) against some respiratory tract pathogens. Science World Journal. 2007;2(2).
  22. Aliyu A, Kevwe BE, Grace U, Samuel EO, Henry OE. High performance liquid chromatography-diode array detection (HPLC-DAD) profiling, antioxidant and anti-proliferative activities of ethanol leaf extract of Berlinia grandiflora (Vahl) Hutch. & Dalziel. Journal of Pharmacognosy and Phytotherapy. 2018;10(11):187-94. https://doi.org/10.5897/JPP2018.0524
  23. Esievo K, Adamu A, Fatokun OT, Chukwuka C, Olatunji KT, Egharevba HO. Phenolic contents, comparative antioxidant studies and anti-tubercular activities of commonly used spices in Abuja, Nigeria. Egyptian Journal of Food Science. 2020;48(2):193-202. 10.21608/ejfs.2020.23810.1041
  24. Jagetia GC, Baliga MS. The evaluation of nitric oxide scavenging activity of certain Indian medicinal plants in vitro: A preliminary study. Journal of Medicinal Food. 2004;7(3):343-48. https://doi.org/10.1089/jmf.2004.7.343
  25. Toxicity–Up AO. OECD guideline for testing of chemicals. 2001.
  26. Sultana R, Khanam S, Devi K. Evaluation of Immunomodulatory activity of Solanum xanthocarpum fruits aqueous extract. Pharm Lett. 2011;3:247-53.
  27. Branch SK. Guidelines from the international conference on harmonisation (ICH). Journal of Pharmaceutical and Biomedical Analysis. 2005;38(5):798-805. https://doi.org/10.1016/j.jpba.2005.02.037
  28. Piechocka J, Gramza-Micha?owska A, Szymandera-Buszka K. The changes in antioxidant activity of selected flavonoids and caffeine depending on the dosage and form of thiamine. Molecules. 2021;26(15):4702. https://doi.org/10.3390/molecules26154702
  29. Lou H, Hu L, Lu H, Wei T, Chen Q. Metabolic engineering of microbial cell factories for biosynthesis of flavonoids: A review. Molecules. 2021;26(15):4522. https://doi.org/10.3390/molecules26154522
  30. Festa J, Da Boit M, Hussain A, Singh H. Potential benefits of berry anthocyanins on vascular function. Molecular Nutrition & Food Research. 2021;65(19):2100170. https://doi.org/10.1002/mnfr.202100170
  31. Hsieh MJ, Huang CY, Kiefer R, Lee SD, Maurya N, Velmurugan BK. Cardiovascular disease and possible ways in which lycopene acts as an efficient cardio-protectant against different cardiovascular risk factors. Molecules. 2022;27(10):3235. https://doi.org/10.3390/molecules27103235
  32. Sohouli M, Haghshenas N, Fatahi S. The association between dietary total antioxidant capacity with the risk of helicobacter pylori infection among adult: a case–control study. 2020.
  33. Lam LM, Murphy S, Kokkinaki D, Venosa A, Sherrill-Mix S, Casu C et al. DNA binding to TLR9 expressed by red blood cells promotes innate immune activation and anemia. Science Translational Medicine. 2021;13(616):eabj1008.
  34. Shruthi S, Vijayalaxmi K, Shenoy KB. Immunomodulatory effects of gallic acid against cyclophosphamide-and cisplatin-induced immunosuppression in Swiss albino mice. Indian Journal of Pharmaceutical Sciences. 2018;80(1):150-60. 10.4172/pharmaceutical-sciences.1000340
  35. Pondugula SR, Harshan A, Ramesh S, Govindarajulu M, Almaghrabi M, Majrashi M et al. Cardioprotective effects of Oroxylum indicum extract against doxorubicin and cyclophosphamide-induced cardiotoxicity. Cardiovascular Toxicology. 2022;22(1):67-77. https://doi.org/10.1007/s12012-021-09701-x
  36. Qi Q, Dong Z, Sun Y, Li S, Zhao Z. Protective effect of bergenin against cyclophosphamide-induced immunosuppression by immunomodulatory effect and antioxidation in Balb/c mice. Molecules. 2018;23(10):2668. https://doi.org/10.3390/molecules23102668
  37. Odumosu P, Lough J, Yakubu D, Thomas K, Williamson G, Haroune N. Anti-mycobacterial assessment and characterization of 5-O-caffeoylquinic acid methyl ester and rutin from Pavetta crassipes. Journal of Applied Pharmaceutical Science. 2016;6(10):001-07. https://doi.org/10.7324/JAPS.2016.601001
  38. John-Samson M. Study of ethnobotany and phytochemistry of Pavetta crassipes leaves and Calotropis procera bark from Malawi. 2012;10.6831/TMU.2012.00101
  39. Rebas E, Rzajew J, Radzik T, Zylinska L. Neuroprotective polyphenols: A modulatory action on neurotransmitter pathways. Current Neuropharmacology. 2020;18(5):431-45. https://doi.org/10.2174/1570159X18666200106155127
  40. Tatipamula VB, Kukavica B. Phenolic compounds as antidiabetic, anti?inflammatory and anticancer agents and improvement of their bioavailability by liposomes. Cell Biochemistry and Function. 2021;39(8):926-44. https://doi.org/10.1002/cbf.3667

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