An anaerobic incubation study was conducted in paddy soil with varying levels of NPK fertilizers. Under submerged conditions, the pH of the soil was greatly influenced by the addition of nitrogen (N) at two different levels: 125 % and 150 % N + recommended phosphorus and potassium (Rec PK). Soil treated with 150 % N + Rec PK resulted in a lower soil pH than other treatments, as evidenced by the notable decrease in soil pH to 6.23 at 14 days after incubation (DAI). When compared to other treatments, 150 % P + Rec NK released more soluble salts at 28 DAI and the highest soil EC value measured was 1.39 dS m-1. With respect to macronutrient availability, the maximum N availability was observed in the 150 % N + Rec PK 28 DAI (236 kg ha-1), while the highest available P was found to be (37.55 mg kg-1) noticed in the 150 % P + Rec NK treatment. The highest available K content was recorded to be 243 kg ha-1 and 234 kg ha-1 in soil treated with 150 % K and 125 % K respectively at 28 DAI. Under anaerobic conditions, a higher release of available NPK was observed between 21 and 28 DAI. Higher nutrient release throughout the incubation period was observed when the NPK level increased to 150%. Increasing the level of NPK to 150 % revealed higher nutrient release during the period of incubation under submerged conditions which can be beneficial for plant grown under nutrient deficit condition.